ALZHEIMER

In vitro

  • Primary cell cultures of neurons with / without astrocytes infected with AAV or LV vectors coding mutated proteins implicated in the pathology
  • Cortical neurons treated with PDC (glutamate transport inhibitor): chronic excitotoxicity model - Follow up of compounds modulating NMDA receptors
  • NGF deprived septal cholinergic neurons: model for neurotrophic compounds
  • Microfluidic compartmentalized neuron cultures: soma is in one compartment and axon in the another one, both compartments being connected to enable amyloid peptide treatment in each compartment
Read-out
Aggregation, propagation in each compartment, cell survival

Cell survival, morphological changes, protein measurement, etc.

Rodents

  • Transgenic mouse models to study factors/drugs targeting amyloidosis (APP/PS1?E9, 3xTg, huAPP)
  • Tauopathies (Rat): lentiviral / AAV – Tau or P301L or Tau fused to a pro-aggregating peptide
  • AAV- hAPP + AAV-hPS1 (mouse and rat)
Read-out
Neuropathological evaluation; 3D histology to evaluate local changes statistically; Local glucose consumption; Brain morphometry using high resolution MRI; Amyloid Plaques by Gadolinium-Stained MRI; Perfusion measurement by MRI and resting state fMRI measures; Behavioral changes; etc.

Aß40, Aß42 and Tau quantification, cognitive function (open field, Morris water maze), LTP, etc.
Non-human Primates
  • Parenchymal inoculation of Alzheimer human brain in Microcebus murinus
  • Tauopathies (Macaques): lentiviral or AAV vectors over-expressing different species of tau proteins (WT, P301L, wild-fused to a pro-aggregating peptide)
Read-out
Tau, Amyloid, Neuroinflammation and glucose metabolism, PET imaging, MRI, MR spectroscopy, histology, motor and cognitive behavior, etc.

Humans

  • Clinical investigation Center (Phase I and II), Biological resource center, stratified cohorts of patients
Read-out
Imagerie par résonance magnétique (IRM), électroencéphalogramme (EEG) haute densité, phénotypage neurologique, neuropsychologique et comportemental, phénotypage immunitaire (FACS), génotypage. 
A comprehensive overview of our offer in the field of Alzheimer's disease:
  • Basic Research

    Cellular models
    In-vitro co-culture systems (primary cell cultures)

    Rat/mouse primary cell cultures of neurons/astrocytes/ or mixed cultures exposed to various mutated proteins implicated in the pathology and delivered either as recombinant proteins or following overexpression through viral vector infection.

    Analyzing Tools

    • Videomicroscopy
    • Flux cytometrye
    • Biochemistry
    • Functional analyses using radioactivity.

  • Preclinical Research
  • Clinical Research